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433 reverse-phase preparative hplc apparatus  (Nacalai)

 
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    Structured Review

    Nacalai 433 reverse-phase preparative hplc apparatus
    433 Reverse Phase Preparative Hplc Apparatus, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse-phase+preparative+hplc+apparatus/reverse+phase+preparative+hplc+apparatus/10__1128_slash_aem__00258___18-217-7-22
    Average 90 stars, based on 1 article reviews
    433 reverse-phase preparative hplc apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (20 by 250 mm) by elution with 40% acetonitrile–water–0.1% trifluoroacetic acid at a flow rate of 5 ml/min at ambient temperature. ..

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The ethyl acetate phase was evaporated to dryness, and the remaining residue was dissolved in a small amount of methanol and purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature. ..

    Article Title: Alkyldihydropyrones, new polyketides synthesized by a type III polyketide synthase from Streptomyces reveromyceticus.
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C18-AR-II column (20 250 mm2; Nacalai Tesque, Kyoto, Japan) by elution with a gradient of methanol in water containing 0.1% trifluoroacetic acid, at a flow rate of 5 ml min 1 at ambient temperature. .. The elution conditions were as follows: 10–70% methanol for 50 min, followed by 70–100% methanol for 40 min. After lyophilization, the compounds were further purified using a recycle HPLC system equipped with a Docosil SP-100 column (10 250 mm; Senshu Scientific).

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature. ..

    High Performance Liquid Chromatography:

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (20 by 250 mm) by elution with 40% acetonitrile–water–0.1% trifluoroacetic acid at a flow rate of 5 ml/min at ambient temperature. ..

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The ethyl acetate phase was evaporated to dryness, and the remaining residue was dissolved in a small amount of methanol and purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature. ..

    Article Title: Alkyldihydropyrones, new polyketides synthesized by a type III polyketide synthase from Streptomyces reveromyceticus.
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C18-AR-II column (20 250 mm2; Nacalai Tesque, Kyoto, Japan) by elution with a gradient of methanol in water containing 0.1% trifluoroacetic acid, at a flow rate of 5 ml min 1 at ambient temperature. .. The elution conditions were as follows: 10–70% methanol for 50 min, followed by 70–100% methanol for 40 min. After lyophilization, the compounds were further purified using a recycle HPLC system equipped with a Docosil SP-100 column (10 250 mm; Senshu Scientific).

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature. ..

    Residue:

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: .. The ethyl acetate phase was evaporated to dryness, and the remaining residue was dissolved in a small amount of methanol and purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature. ..

    other:

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
    Article Snippet: The crude materials were purified using 414 a reverse-phase preparative HPLC apparatus equipped with a 5C18-AR-II column (10 × 415 250 mm; Nacalai Tesque) by elution with 30% methanol in water containing 0.1% 416 trifluoroacetic acid at a flow rate of 3 mL/min at ambient temperature.



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    <t>HPLC</t> chromatograms of the recombinant Streptomyces strains. Chromatograms of strains expressing SoceCHS1 (A); SoceCHS1 and BdsA (B); SoceCHS1, BdsA, and BdsB (C); BdsA and BdsB (E); BdsA (F); and BdsB (G) are shown. (D) Chromatogram of authentic compound 1. (H) Chromatogram of the strain carrying an empty vector. AU, absorbance units.
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    <t>HPLC</t> chromatograms of the recombinant Streptomyces strains. Chromatograms of strains expressing SoceCHS1 (A); SoceCHS1 and BdsA (B); SoceCHS1, BdsA, and BdsB (C); BdsA and BdsB (E); BdsA (F); and BdsB (G) are shown. (D) Chromatogram of authentic compound 1. (H) Chromatogram of the strain carrying an empty vector. AU, absorbance units.
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    Image Search Results


    HPLC chromatograms of the recombinant Streptomyces strains. Chromatograms of strains expressing SoceCHS1 (A); SoceCHS1 and BdsA (B); SoceCHS1, BdsA, and BdsB (C); BdsA and BdsB (E); BdsA (F); and BdsB (G) are shown. (D) Chromatogram of authentic compound 1. (H) Chromatogram of the strain carrying an empty vector. AU, absorbance units.

    Journal: Applied and Environmental Microbiology

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum

    doi: 10.1128/AEM.00258-18

    Figure Lengend Snippet: HPLC chromatograms of the recombinant Streptomyces strains. Chromatograms of strains expressing SoceCHS1 (A); SoceCHS1 and BdsA (B); SoceCHS1, BdsA, and BdsB (C); BdsA and BdsB (E); BdsA (F); and BdsB (G) are shown. (D) Chromatogram of authentic compound 1. (H) Chromatogram of the strain carrying an empty vector. AU, absorbance units.

    Article Snippet: The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature.

    Techniques: Recombinant, Expressing, Plasmid Preparation

    Catalytic properties of BdsA. (A) HPLC chromatograms representing BdsA reactions. The enzyme, substrate, and cofactor used are shown at the top right of the chromatograms. (B) Michaelis-Menten saturation curve of BdsA.

    Journal: Applied and Environmental Microbiology

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum

    doi: 10.1128/AEM.00258-18

    Figure Lengend Snippet: Catalytic properties of BdsA. (A) HPLC chromatograms representing BdsA reactions. The enzyme, substrate, and cofactor used are shown at the top right of the chromatograms. (B) Michaelis-Menten saturation curve of BdsA.

    Article Snippet: The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature.

    Techniques:

    Catalytic properties of BdsB. (A) HPLC chromatograms representing BdsB reactions. The enzyme and substrate used are shown at the top right of the chromatograms. (B) Michaelis-Menten saturation curves of BdsB.

    Journal: Applied and Environmental Microbiology

    Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum

    doi: 10.1128/AEM.00258-18

    Figure Lengend Snippet: Catalytic properties of BdsB. (A) HPLC chromatograms representing BdsB reactions. The enzyme and substrate used are shown at the top right of the chromatograms. (B) Michaelis-Menten saturation curves of BdsB.

    Article Snippet: The crude materials were purified using a reverse-phase preparative HPLC apparatus equipped with a 5C 18 -AR-II column (Nacalai Tesque) (10 by 250 mm) by elution with 30% methanol–water–0.1% trifluoroacetic acid at a flow rate of 3 ml/min at ambient temperature.

    Techniques: